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TURKISH REPUBLIC ISTANBUL UNIVERSITY ISTANBUL FACULTY OF MEDICINE Department of Microbiology and Clinical Microbiology TO WHOM IT MAY CONCERN |
"VIRUSSAFE MEDICAL AIR PURIFIER" branded Filteriess Plasma Ion Generator- Air Sterilization Equipment's affectiveness is tested by our Head Microbiological Department Laboratories by request of Equipment manufacturer Nero Industries LTD.
Test Startup Date: 22.11.2010 Test Termination Date: 20.01.2011
Test Location: Istanbul Faculty of Medicine, Department of Microbiology and Clinical Microbiology
Test Material : VIRUSSAFE MEDICAL brand, Filteriess Plasma Ion Generator, Model- Air Sterilization Equipment
Test Equipments : 1 m3 Volume Isolated Test Chamber with Solution Diffuser and Safe Reach Accessones.
Test Procedure : The test based on sterilization of the air contaminated with different bacterial strains. For this purpose, 1 m3 volumetnc isolated test chamber was performed. One Virussafe Medical Air Purifier Unit was placed on the floor of the chamber. Bioburden of environmental air with bacteria was mesured before getting in action with VIRUSSAFE MEDICAL Equipment. The Petri dishes were opened and collected by the using sterile hand gloves. The test was conducted by opening Petri dishes containing bacterial media previously prepared on the floor of the chamber followed by pulverization of 100 ml of bacterial suspension containing 10s CFU bactena/ml density for 5 minutes to chamber air. The following bactena were used; Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa NCTC 6749, Escherichia co/i ATCC 11229, Bacillussubtilis varniger ATCC9372. The equipment was started up and at the end of the tested times; 5 , 15, 30 ,and 60 minutes, all Petri dishes were collected and incubated at 35 °C for 48 hours. The ratio of change in bacetial colony number (amount) CFU was recorded and calculated accordingly. The experiments were repeated 3 times and the mean number was shown in the table below.
Table 1: Bacteria Colony Count and Reduction Data
Bacterial culture in Petri dish |
S.aureus ATCC 6538 Activation time (minutes)
0 5 60 |
P.aeruginosa NCTC 6749 Astivattofl time (minutes)
0 5 60 |
E.coli ATCC 11229 Activation time immuJMgl
0 5 60 |
B. subtilis var niger ATCC 9372 Activation time
(minutes)
0 5 60 |
Colony Forming Unit (cfu) Quantity |
1333 |
230 |
113 |
163 |
6 |
- |
51 |
44 |
2 |
186 |
36 |
20 |
% Reduction |
0 |
82.70 |
91.50 |
0 |
96.30 |
99.99 |
0 |
86.53 |
91.15 |
0 |
80.70 |
89.30 |
As seen on the table, the equipment performed sterilization on the tested bacteria as %82,7 to %96,3 in five (5) minutes and % 91.5-% 99.99 sterilization in 60 minutes.
"VIRUSSAFE MEDICAL AIR PURIFIER" brand , Filteriess Plasma Ion Generator- Air Sterilization Equipment's performance is recorded and approved on the air contaminated with pathogenic microorganisms, thus, playing an important role in reduction of epidemic infection risk and have preventation usage.

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